ABSTRACT
Objective To investigate the miR-132 expression in different segments of the nervous system through mechanical damage induced neuropathic pain rat model. Methods A total of 45 male SD rats(250~300 g)were randomly divided into Native,Shame and BCCI groups,with 15 rats in each group. The mechanical with-drawal threshold(MWT)was measured 1 day before BCCI,and 1 d,7 d,14 d after BCCI. The expression levels of miR-132 in Hippocampus(HP),L4~6 spinal dorsal horn(SDH)and dorsal root ganglia(DRG)were measured indi-vidually by reverse transcription-polymerase chain reaction(RT-PCR). Results Compared with Shame group and Native group,MWT decreased significantly in BCCI group 7 d and 14 d after operation(P<0.01). Compared with the Native group,the expression of miR-132 in DRG decreased in BCCI group and Shame group 7 d after operation (P < 0.05). Rats in shame group recovered by 14 d while BCCI was still decreased significantly(P < 0.05). The miR-132 expression of SDH was upregulated significantly in BCCI group at day 7(P<0.05). On the 14 day after operation,the expression of miR-132 was downregulated significantly in BCCI group and Shame group(P<0.05). BCCI group decreased significantly at 14 d(P < 0.01). Conclusions MiR-132 was involved in the regulation of neuropathic pain. The expression of miR-132 has its own characteristics in different segments and time points.
ABSTRACT
Objective To investigate the miR-132 expression in different segments of the nervous system through mechanical damage induced neuropathic pain rat model. Methods A total of 45 male SD rats(250~300 g)were randomly divided into Native,Shame and BCCI groups,with 15 rats in each group. The mechanical with-drawal threshold(MWT)was measured 1 day before BCCI,and 1 d,7 d,14 d after BCCI. The expression levels of miR-132 in Hippocampus(HP),L4~6 spinal dorsal horn(SDH)and dorsal root ganglia(DRG)were measured indi-vidually by reverse transcription-polymerase chain reaction(RT-PCR). Results Compared with Shame group and Native group,MWT decreased significantly in BCCI group 7 d and 14 d after operation(P<0.01). Compared with the Native group,the expression of miR-132 in DRG decreased in BCCI group and Shame group 7 d after operation (P < 0.05). Rats in shame group recovered by 14 d while BCCI was still decreased significantly(P < 0.05). The miR-132 expression of SDH was upregulated significantly in BCCI group at day 7(P<0.05). On the 14 day after operation,the expression of miR-132 was downregulated significantly in BCCI group and Shame group(P<0.05). BCCI group decreased significantly at 14 d(P < 0.01). Conclusions MiR-132 was involved in the regulation of neuropathic pain. The expression of miR-132 has its own characteristics in different segments and time points.